A man and a woman died in the shooting, said interim police chief Mark Simmons.
The 14 injured people were taken to two different hospitals, Simmons said, noting that none of the injured were at risk of death.
“This is truly a tragedy of epic proportions,” said Simmons near the crime scene.
The shooting comes at a time when the city’s police department was shaken by Daniel Prude’s asphyxiation death.
A video filmed in March and made public by Prude’s family on September 4 shows Prude handcuffed and naked, while one agent pushes his face against the floor in the street while another agent presses a knee on his back.
The officers pushed him against the floor for about two minutes until he stopped breathing.
A week later, life support was withdrawn from Prude.
On Monday, mayor Lovely Warren fired police chief La’Ron Singletary, claiming that he initially misled her about the circumstances of Prude’s death.